AbCap A 4FF

Smart-Lifesciences rProtein A Beads 4FF is an affinity chromatography medium designed for the purification of monoclonal antibody, polyclonal antibody and Fc tag fusion protein.The recombinant protein A is coupled to highly cross-linked 4% sepharose gel. This coupling method can improve the binding capacity for immunoglobulin. The static binding capacity of rProtein A Beads 4FF is greater than 40 mg human IgG/ml settled beads. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc. The characteristics of rProtein A Beads 4FF are summarized in Table  . 


Protein A ,a bacterial cell wall protein isolated from Staphylococcus aureus, combines mammalian IgGs mainly through Fc regions. Recombinant protein A contains five high affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding. 


AbCap A 4FF is one of a range of prepacked, ready-to-use columns for affinity chromatography. It is packed with 1 ml and 5 ml of rProtein A Beads 4FF. Five different packing sizes are available. AbCap A 4FF has the standard interface and can be adapted to all kinds of chromatography system, such as ÄKTA. It is fast, simple and easy operation.



  • Product Performance
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• Product Description

ItemDescription
Matrix SphericalHighly cross-linked 4% agarose
LigandRecombinant Protein A
Static binding capacity>40 mg Human lgG/ml medium
Particle size45-165 μm
Maxi Pressure0.3 MPa, 3 bar
PH stability3-10
Storage solution1X PBS containing 20% ethanol
Storage temperature2 – 8℃


• Related Products

ProductCat.No.Size
AbCap A 4FFSA015C111 X 1 ml
SA015C515 X 1 ml
SA015C151 X 5 ml
SA015C555 X 5 ml
SA015CS3X1 ml+1X5 ml


• Download Instructions

AbCap A 4FF English.pdf

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