Changzhou Smart-Lifesciences Biotechnology Co.,Ltd.

Plasmid has wide applications in biotechnology and are crucial in the mRNA production process. Plasmid extraction is typically performed from E. coli after fermentation. Initially, the cell pellet must be lysed using methods such as freeze-thaw lysis, alkaline lysis, or ultrasonic fragmentation. Following 6FF chromatography, most RNA is removed. High-purity covalently closed circular DNA (cccDNA) is then obtained using Smac PlasmidSelect affinity resin. Finally, strong anion exchange chromatography is used to reduce endotoxin levels, ensuring the process meets drug registration standards.