Smart-Lifesciences rProtein A Beads is an affinity chromatography medium designed for the purification of monoclonal antibody, polyclonal antibody and Fc tag fusion protein.The recombinant protein A is coupled to 4% sepharose gel. This coupling method can improve the binding capacity for immunoglobulin. The static binding capacity of rProtein A Beads is greater than 40 mg human IgG/ml settled beads. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc. The characteristics of rProtein A Beads are summarized in Table .
Protein A ,a bacterial cell wall protein isolated from Staphylococcus aureus, combines mammalian IgGs mainly through Fc regions. Recombinant protein A contains five high affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding.
AbPur rProtein A Kit provides 3 ml rProtein A Beads gravity prepacked column and buffer required for antibody purification, which is convenient for customers to use, simple to operate and high in purification efficiency. The components of the kit are shown in the table below.
• Product Description
| Item | Description |
| Matrix | 4% agarose |
| Ligand | Recombinant Protein A |
| Loading capacity | > 40 mg human IgG/ml medium |
| Particle size range | 45-165 μm |
| Maxi pressure | 0.1 MPa, 1 bar |
| PH stability | 3-10 |
| Storage buffer | 1×PBS containing 20% ethanol |
| Storage temperature | 2 – 8℃ |
• Components
| Component Name | Cat.No. | Size | Quantity |
| AbPur rProtein A Column | SA012GC03 | 3 ml | 1 |
| Ab Binding/Wash Buffer | SLB017100 | 100 ml | 2 |
| Ab Elution Buffer | SLB018100 | 100 ml | 1 |
• Ordering Information
| Product | Cat.No. | Size |
| AbPur rProtein A Kit | SA012K03 | 3T |
• Download Instructions
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