Changzhou Smart-Lifesciences Biotechnology Co.,Ltd.

rProtein G Beads is an affinity chromatography medium designed for purification of monoclonal antibody, polyclonal antibody and Fc tag fusion protein. The recombinant protein G ligand is coupled to 4% agarose beads. The Characteristics of rProtein G Beads are summarized in Table .

Protein G, a bacterial cell wall protein isolated from group G Streptococci, binds to mammalian IgGs mainly through Fc regions. Native protein G has 3 IgG binding domains and also sites for albumin and cell-surface binding. The recombinant protein G have been eliminated the nonspecific binding site. Although protein G has very similar tertiary structures to protein A, their amino acid compositions differ significantly, resulting in different binding characteristics. Protein G can be used for purification of mammalian monoclonal and polyclonal IgGs that do not bind well to protein A. Protein G has greater affinity than protein A for most mammalian IgGs, especially for certain subclasses including human IgG3, mouse IgG1 and rat IgG2a.   

AbPur rProtein G Kit provides 3 ml rProtein G Beads gravity prepacked column and buffer required for antibody purification, which is convenient for customers to use, simple to operate and high in purification efficiency. The detailed components of the kit are shown in the table below.