The RFP (Red fluorecent protein) is a molecular marker isolated from anemone and is often used to study protein localization, reporter gene expression and protein-protein interaction. The agarose bead surface is coated with anti-RFP antibody, a highly specific mouse monoclonal antibody that can be used for immunoprecipitation, co-immunoprecipitation, affinity chromatography of natural RFP, RFP mutants and their fusion proteins. Characteristics of Anti-RFP Affinity Beads 4FF: 1) The ligand antibody has a very high affinity with RFP, which can efficiently capture RFP tagged proteins in the cell lysis; 2) After immunoprecipitation, SDS-PAGE test has a cleaner background and is more conducive to the identification of protein interactions. 3) Agarose material is characterized by low non-specific adsorption and good compatibility, with a capacity of more than 1 mg RFP fusion protein/ml (up to 5 mg/ml), and can be used for the massive purification of RFP fusion proteins.
• Product Description
| Item | Description |
| Matrix Spherical | 4% agarose |
| Ligand | Anti-RFP Antibody |
| Static binding capacity | >1mg RFP fusion protein/ml medium |
| Particle size | 45-165 μm |
| Maxi pressure | 0.3 MPa,3 bar |
| Storage solution | 1×PBS containing 0.02% NaN3 |
| Storage temperature | 2- 8℃ |
• Related Products
| Product | Cat.No. | Size |
| Anti-RFP Affinity Beads 4FF | SA072001 | 1 ml |
| SA072005 | 5 ml | |
| SA072025 | 25 ml | |
| SA072100 | 100 ml | |
| SA072500 | 500 ml | |
| SA07201L | 1 L |
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