Cu IDA Beads can be used to purify 6xHis-tagged proteins expressed in series of expression systems, such as E.coli., yeast, insect cells and mammalian cells. Cu IDA Beads consists of 90µm beads of 4% agarose, to which iminodiacetic acid (IDA) has been coupled. The chelating group has then been charged with copper ions (Cu2+). This form is relatively stable plane structure, which can be destroyed by other competitive small molecule. The structure of Cu-IDA has more sites to bind histidine-tagged proteins. Cu IDA Beads has the advantages of higher binding capacity and cost-effective.
• Product Description
| Item | Description |
| Matrix | 4% agarose |
| Loading capacity | ＞20 mg 6×His-tagged protein/ml medium |
| Particle size | 45-165 μm |
| Maxi pressure | 0.1 MPa, 1 bar |
| Storage buffer | 1×PBS containing 20% ethanol |
| Storage temperature | 4-30℃ |
• Related Products
| Product | Cat.No. | Size |
| Cu IDA Beads | SA041005 | 5 ml |
| SA041025 | 25 ml | |
| SA041100 | 100 ml | |
| SA041500 | 500 ml | |
| SA04101L | 1 L | |
| SA04110L | 10 L |
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