Cu IDA Beads can be used to purify 6xHis-tagged proteins expressed in series of expression systems, such as E.coli., yeast, insect cells and mammalian cells. Cu IDA Beads consists of 90µm beads of 4% agarose, to which iminodiacetic acid (IDA) has been coupled. The chelating group has then been charged with copper ions (Cu2+). This form is relatively stable plane structure, which can be destroyed by other competitive small molecule. The structure of Cu-IDA has more sites to bind histidine-tagged proteins. Cu IDA Beads has the advantages of higher binding capacity and cost-effective.
Cu IDA Beads Gravity Column are packed with 1ml and 5ml of Cu IDA Beads . It is easy to operate.
• Product Description
| Item | Description |
| Matrix | 4% agarose |
| Loading capacity | >20 mg 6×His-tagged protein/ml medium |
| Particle size | 45-165 μm |
| Maxi pressure | 0.1 MPa, 1 bar |
| Storage buffer | 1×PBS containing 20% ethanol |
| Storage temperature | 2-8℃ |
• Related Products
| Product | Cat.No. | Size |
| Cu IDA Beads Gravity Column | SA041GC01 | 1 ml |
| SA041GC05 | 5 ml |
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