Protein At Beads LX is a novel, alkali-tolerant protein A-derived medium for capturing monoclonal antibodies from larger volumes of feed by packed bed chromatography. Table is Characteristics of Protein At Beads LX. Alkali-tolerant protein A (protein At) is obtained by gene recombination technology on the base of natural protein A. The ligand has no animal origin during purification. The ligand is specially designed to enhance its stability to alkali and protease.Protein At Beads LX has high dynamic binding capacity (DBC) after extended retention time and is specially designed for process feed from high-expression cell cultures with increased antibody titers.
Ligand is directional immobilized to the agarose beads. The level of leakage of the ligand during elution is very low (less than 10ng ligand/mg antibody).
The base matrix of Protein At Beads LX is 85 µm beads of highly cross-linked agarose derivative with excellent chemical and physical stabilities, making it ideal for process scale applications.
HiPur At LX is a prepacked ready to use column. It is packed with 20ml Protein At Beads LX .The arrow on the column label indicates the recommended flow direction.HiPur At LX can be adapted to all kinds of chromatography system, such as ÄKTA. It is easy to operate.
• Product Description
| Item | Description |
| Specification | 20ml |
| Matrix | Highly cross-linked agarose beads |
| Ligand | Protein At (Alkali-tolerant protein A) |
| Static Binding Capacity | >60 mg Human lgG/ml medium |
| Particle size | ~85 μm |
| Chemical stability | all reagent in the process of antibody purification |
| Cleaning-in-place | 0.1-0.5 M NaOH |
| Column size (inner diameter x height) | 1.6×10cm |
| Recommended flow rate | 50-500 cm/h |
| Storage | 1XPBS with 20% ethanol, 2 – 8°C |
• Related Products
| Product | Cat.No. | Size |
| HiPur At LX | SA085C20 | 20ml |
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