Con A Beads 4FF is an affinity chromatography medium that conjugates Con A with agarose to purify some glycoproteins. Con A is a plant haemagglutinin separated from giant bean(Jack bean, Canavalia ensiformis),which can binds ɑ - D - pyran mannose, ɑ - D - pyran on glucose and Molecular groups related to its space position of the combination.Con A binds to carbohydrate molecules mainly in the hydroxyl part of C-3, C-4 and C-6. Con A combines better with D-pyranoid mannose than with D-pyranosaccharide.Con A Beads 4FF is used to isolate and purify some glycoproteins, membrane proteins, sugar lipids, polysaccharides, membrane vesicles with mannitin or glucoside residues, lgM, hormone lipoproteins and so on. Substances such as trypsin inhibitors in human serum, alkaline phosphatase, calf spleen phosphodiesterase, various variants of alpha-fetoglobulin and certain hormones such as chorionic gonadotropin (HCG) and luteinizing hormone (LH) can be purified with it.
HiPur ConA 4FF is a prepacked ready to use column. It is packed with 20ml Con A Beads 4FF .The arrow on the column label indicates the recommended flow direction.HiPur ConA 4FFcan be adapted to all kinds of chromatography system, such as ÄKTA. It is easy to operate.
• Product Description
| Item | Description |
| Size | 20 ml |
| Matrix | Highly cross-linked 4% agarose |
| Ligand | Con A |
| Ligand density | >20 mg thyroglobulin /ml medium |
| Particle size range | 45-165 μm |
| Maxi pressure | 0.3 MPa, 3 bar |
| pH stability | 4-9 |
| Column size | 1.6×10 cm |
| Storage buffer | 0.1 M acetate,1 MNaCl,1 mMCaCl2,1 mM MnCl2,1 mM MgCl2,20% ethanol,pH6.0 |
| Storage temperature | 2 – 8℃ |
• Related Products
| Product | Cat.No. | Size |
| HiPur ConA 4FF | SA028C20 | 20 ml |
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