SmartCore 700 is aimed at intermediate purification and polishing of viruses and other large biomolecules in flow through mode. Each Bead has a ligand-activated core and an outer layer without ligands. It prevents larger targets from entering the beads, while smaller proteins and impurities bind to the hydrophobic and positively charged octylamine ligand in the core. The molecular size cut-off for proteins is approximately Mr=690000.
HiPur SmartCore 700 is a prepacked ready to use column. It is packed with 20ml SmartCore 700.The arrow on the column label indicates the recommended flow direction.HiPur SmartCore 700can be adapted to all kinds of chromatography system, such as ÄKTA. It is easy to operate.
• Product Description
| Item | Description | |||
| Specification | 20ml | |||
| Matrix | polymer microspheres | |||
| Ligand | Octylamine | |||
| Dynamic binding capacity | 15-20 mg BSA/mL(5%flow through point) | |||
| Ion exchange capacity | 0.15-0.30 mmol/ml | |||
| Average particle size | 90 μm | |||
| Maxi pressure | 1.5 MPa | |||
| pH stability | 1-14 | |||
| Cleaning and regeneration | 1 M NaOH+30% Isopropyl alcohol | |||
| Column size | 1.6×10cm | |||
| Storage buffer | 20% ethanol | |||
| Storage temperature | 4 – 30°C | |||
| Chemical stability | common purification solutions,1M NaOH,6M guanidine hydrochloride、30%Isopropyl alcohol、70%ethanol | |||
• Related Products
| Product | Cat.No. | Size |
| HiPur SmartCore 700 | SEC022C20 | 20 ml |
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