Changzhou Smart-Lifesciences Biotechnology Co.,Ltd.

Smart-Lifesciences rProtein A Beads 4FF is an affinity chromatography medium designed for the purification of monoclonal antibody, polyclonal antibody and Fc tag fusion protein.The recombinant protein A is coupled to highly cross-linked 4% sepharose gel. This coupling method can improve the binding capacity for immunoglobulin. The static binding capacity of rProtein A Beads 4FF is greater than 40 mg human IgG/ml settled beads. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc. The characteristics of rProtein A Beads 4FF are summarized in Table  . 

Protein A ,a bacterial cell wall protein isolated from Staphylococcus aureus, combines mammalian IgGs mainly through Fc regions. Recombinant protein A contains five high affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding. 

HiSelect A 4FF is a prepacked ready to use column. It is packed with 4.7ml rProtein A Beads 4FF .The arrow on the column label indicates the recommended flow direction.HiSelect A 4FF can be adapted to all kinds of chromatography system, such as ÄKTA. It is easy to operate.