Glutathione Beads 4FF is an affinity chromatography medium designed for the purification of glutathione S-transferase (GST)-tagged proteins produced using the pGEX series of expression vectors, other glutathione S-transferases and glutathione binding proteins.
The glutathione ligand is coupled to highly cross-linked 4% agarose beads. The coupling is optimized to give high binding capacity for GST-tagged proteins and other glutathione binding proteins. Glutathione Beads 4FF can purify GST-tagged proteins under high flow rate. Its high flow properties make it excellent for scale-up. The characteristics of Glutathione Beads 4FF are summarized in Table .
HiSelect Glutathione 4FF is a prepacked ready to use column. It is packed with 4.7ml Glutathione Beads 4FF .The arrow on the column label indicates the recommended flow direction.HiSelect Glutathione 4FF can be adapted to all kinds of chromatography system, such as ÄKTA. It is easy to operate.
• Product Description
| Item | Description | |
| Size | 4.7ml | |
| Column size | 0.77×10 cm | |
| Matrix spherical | highly cross-linked 4% agarose | |
| Ligand | Reduced Glutathione | |
| Static binding capacity | >10 mg GST-tagged protein /ml medium | |
| Particle size | 45–165 μm | |
| Maxi pressure | 0.3 MPa,3 bar | |
| PH stability | 3-12 | |
| Storage solution | 1×PBS containing 20% ethanol | |
| Storage temperature | 2-8℃ | |
• Related Products
| Product | Cat.No. | Size |
| HiSelect Glutathione 4FF | SA010C47 | 4.7ml |
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