rProtein L Beads 4FF is an affinity chromatography medium designed for purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. The recombinant protein L ligand is immobilized to highly cross-linked 4% agarose beads. The Characteristics of rProtein L Beads 4FF are summarized in Table.
Protein L is recombinantly expressed in E. coli and retains the property of binding to the antibody κ-chain without affecting the antigenic binding site of the antibody. Protein L binds well to the kappa light chain of human and mouse, and may bind specifically to certain kappa isoforms of other species. Protein L binds weakly to rabbit immunoglobulins and does not bind bovine, goat or sheep source immunoglobulins.
HiSelect L 4FF is a prepacked ready to use column. It is packed with 4.7ml rProtein L Beads 4FF .The arrow on the column label indicates the recommended flow direction.HiSelect L 4FFcan be adapted to all kinds of chromatography system, such as ÄKTA. It is easy to operate.
• Product Description
| Item | Description |
| Specification | 4.7ml |
| Matrix spherical | Highly cross-linked 4% agarose |
| Ligand | recombinant protein L |
| Static binding capacity | ＞15mg Human lgG/ml medium |
| Particle size | 45-165 μm |
| Maxi Pressure | 0.3 MPa, 3 bar |
| PH stability | 3-10 |
| Column size (inner diameter x height) | 0.77×10cm |
| Storage solution | 1XPBS containing 20% ethanol |
| Storage temperature | 2 – 8℃ |
• Related Products
| Product | Cat.No. | Size |
| HiSelect L 4FF | SA033C47 | 4.7ml |
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