HiSelect Ni Smart from China Manufacturer

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HiSelect Ni Smart

Ni Smart Beads 6FF is a new immobilized metal ion affinity chromatography(IMAC) medium precharged with nickel ions. Ni Smart Beads 6FF is designed mainly for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants. The strong nickel ion binding also provides very high resistance to EDTA and reducing agents like DTT. Ni Smart Beads 6FF enable direct loading of large sample volumes without having to remove agents that cause stripping of nickel ions from conventional IMAC medium. Ni Smart Beads 6FF is stable in all buffers commonly used in IMAC.

HiSelect Ni Smart is a prepacked ready to use column. It is packed with 4.7ml Ni Smart Beads 6FF.The arrow on the column label indicates the recommended flow direction. HiSelect Ni Smart can be adapted to all kinds of chromatography system, such as ÄKTA. It is easy to operate.

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• Product Description

Item	Description
Specification	4.7ml
Matrix	Highly cross-linked 6% agarose
Loading capacity	＞10 mg 6×His-tagged protein/ml medium
Particle size	45-165 μm
Maxi pressure	0.3 MPa, 3 bar
Column size (inner diameter x height)	0.77×10cm
Storage buffer	1× PBS containing 20% ethanol
Storage temperature	2-8℃