Recombinant streptavidin isolated from Streptomyces avidinii is immobilized on high-crossed linked 6% agarose beads. Streptavidin Beads 6FF can be used to bind biotin and biotinylated substances. The interaction between streptavidin and biotin is very strong and requires denaturating conditions for elution, which may destroy both the ligand and the sample. The interaction between 2-iminobiotin and streptavidin is weak, it can be eluted at pH4.0, which preserve the biomolecules activity.The cross-linking of the base matrix has been optimized to give the matrix good flow properties and high physical and chemical stability, both of which are key factors for cost-effective, large-scale use.
HiSelect Streptavidin 6FF is a prepacked ready to use column. It is packed with 4.7ml Streptavidin Beads 6FF .The arrow on the column label indicates the recommended flow direction.HiSelect Streptavidin 6FF can be adapted to all kinds of chromatography system, such as ÄKTA. It is easy to operate.
• Product Description
| Item | Description | |
| Size | 4.7ml | |
| Column size | 0.77×10 cm | |
| Matrix | Highly cross-linked 6% agarose | |
| Ligand | Streptavidin | |
| Capacity | >200nmol Biotin/ml medium | |
| Particle size | 45-165 μm | |
| Maxi pressure | 0.3 MPa, 3 bar | |
| PH stability | 2-9 | |
| Srorage buffer | 1×PBS containing 20% ethanol | |
| Storage temperature | 2 – 8℃ | |
• Related Products
| Product | Cat.No. | Size |
| HiSelect Streptavidin 6FF | SA021C47 | 4.7ml |
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