Ni Smart Beads 6FF is a new immobilized metal ion affinity chromatography(IMAC) medium precharged with nickel ions. Ni Smart Beads 6FF is designed mainly for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants. The strong nickel ion binding also provides very high resistance to EDTA and reducing agents like DTT. Ni Smart Beads 6FF enable direct loading of large sample volumes without having to remove agents that cause stripping of nickel ions from conventional IMAC medium. Ni Smart Beads 6FF is stable in all buffers commonly used in IMAC.
HisCap Smart 6FF is one of a range of prepacked, ready-to-use columns for affinity chromatography. It is packed with 1 ml and 5 ml of Ni Smart Beads 6FF. Five different packing sizes are available. HisCap Smart 6FF has the standard interface and can be adapted to all kinds of chromatography system, such as ÄKTA. It is fast, simple and easy operation.
• Product Description
| Item | Description | |
| Specification | 1 ml | 5 ml |
| Matrix | Highly cross-linked 6% agarose gel | |
| Loading capacity | ＞10 mg 6×His-tagged protein/ml medium | |
| Particle size | 45-165 μm | |
Maxi pressure | 0.3 MPa, 3 bar | |
| Column size (inner diameter x height) | 0.7×2.5 cm | 1.6×2.5 cm |
| Storage buffer | 1× PBS containing 20% ethanol | |
| Storage temperature | 2-8℃ | |
• Related Products
| Product | Cat.No. | Size |
| HisCap Smart 6FF | SA036C11 | 1×1 ml |
| SA036C51 | 5×1 ml | |
| SA036C15 | 1×5 ml | |
| SA036C55 | 5×5 ml | |
| SA036CS | 3×1 ml+1×5 ml |
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