Changzhou Smart-Lifesciences Biotechnology Co.,Ltd.

Ni NTA Beads can be used to purify 6xHis-tagged proteins expressed in series of expression vectors, such as E.coli., yeast, insect cells and mammalian cells. Ni NTA Beads consists of 90 µm beads of 4% agarose, to which nitrilotriacetic acid (NTA) has been coupled. The chelating group has then been charged with nickel ions (Ni2+). This form is very stable octahedral structure of nickel ions in the center, which can protect the nickel ions from attack of the competitive small molecule . The structure of Ni NTA Beads is compatible with a certain concentration of reducing agents, denaturing agents, detergents and other additives. Ni NTA Beads has wider applicability, more stable ligand and higher selectivity than that of Ni IDA Beads.

The HisPur Ni NTA kit provides 3ml Ni NTA Beads gravity prepacked column and the buffer required for histidine tag protein purification, which is convenient for customers to use, simple to operate and high in purification efficiency. The detailed components of the kit are shown in Table  below.