Ni IDA Beads from China Manufacturer

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Ni IDA Beads

Ni IDA Beads can be used to purify 6xHis-tagged proteins expressed in series of expression Systems, such as E.coli., yeast, insect cells and mammalian cells. Ni IDA Beads consists of 90 µm beads of 4% agarose, to which iminodiacetic acid (IDA) has been coupled. The chelating group has then been charged with nickel ions (Ni²⁺). This form is relatively stable plane structure, which can be destroyed by other competitive small molecule. The structure of Ni-IDA has more sites to bind histidine-tagged proteins. Ni IDA Beads has the advantages of higher binding capacity and cost-effective.

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• Product Description

Item	Description
Matrix	4% agarose
Static binding capacity	＞40 mg 6×His-tagged protein/ml medium
Particle size	45–165μm
Maxi pressure	0.1 MPa, 1 bar
Storage Solution	1XPBS containing 20% ethanol
Storage temperature	4°C-30°C