Ni NTA Beads

Ni NTA Beads can be used to purify 6xHis-tagged proteins expressed in series of expression vectors, such as E.coli., yeast, insect cells and mammalian cells. Ni NTA Beads consists of 90 µm beads of 4% agarose, to which nitrilotriacetic acid (NTA) has been coupled. The chelating group has then been charged with nickel ions (Ni2+). This form is very stable octahedral structure of nickel ions in the center, which can protect the nickel ions from attack of the competitive small molecule . The structure of Ni NTA Beads is compatible with a certain concentration of reducing agents, denaturing agents, detergents and other additives. Ni NTA Beads has wider applicability, more stable ligand and higher selectivity than that of Ni IDA Beads.

  • Product Performance
  • Ordering Information
  • Download Manual

• Product Description

ItemDescription
Matrix Spherical4% agarose
Static binding capacity>40 mg 6×His-tagged protein/ml medium
Particle size45-165 μm
Maxi pressure0.1 MPa, 1 bar
Storage Solution1× PBS containing 20% ethanol
Storage temperature4-30℃


• Related Products

ProductCat.No.Size
Ni NTA BeadsSA0040055 ml
SA00402525 ml
SA004100100ml
SA004500500ml
SA00401L1L
SA00410L10L
HisPur Ni NTA KitSA004K033 times
Ni NTA Beads 6FFSA0050055 ml
SA00502525 ml
SA005100100ml
SA005500500ml
SA00501L1L
SA00510L10L
HisCap 6FF
SA005C111×1 ml
SA005C515×1 ml
SA005C151×5 ml
SA005C555×5 ml
SA005CS3×1 ml+1×5 ml


• Download Instructions

Ni NTA Beads English 2024.09.pdf


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