Ni NTA Beads Gravity Column from China Manufacturer

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Ni NTA Beads Gravity Column

Ni NTA Beads can be used to purify 6xHis-tagged proteins expressed in series of expression vectors, such as E.coli., yeast, insect cells and mammalian cells. Ni NTA Beads consists of 90 µm beads of 4% agarose, to which nitrilotriacetic acid (NTA) has been coupled. The chelating group has then been charged with nickel ions (Ni²⁺). This form is very stable octahedral structure of nickel ions in the center, which can protect the nickel ions from attack of the competitive small molecule. The structure of Ni NTA Beads is compatible with a certain concentration of reducing agents, denaturing agents, detergents and other additives. Ni NTA Beads has wider applicability, more stable ligand and higher selectivity than that of Ni IDA Beads.

Ni NTA Beads Gravity Column are packed with 1ml and 5ml of Ni NTA Beads. It is easy to operate.

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• Product Description

Item	Description
Matrix	4% agarose
Loading capacity	＞40 mg 6×His-tagged Protein/ml matrix
Microsphere particle size	45-165 μm
Maxi pressure	0.1 MPa, 1 bar
Storage buffer	1× PBS containing 20% ethanol
Storage temperature	2-8℃