Ni Smart Beads 6FF Gravity Column

Ni Smart Beads 6FF is a new immobilized metal ion affinity chromatography(IMAC) medium precharged with nickel ions. Ni Smart Beads 6FF is designed mainly for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants. The strong nickel ion binding also provides very high resistance to EDTA and reducing agents like DTT. Ni Smart Beads 6FF enable direct loading of large sample volumes without having to remove agents that cause stripping of nickel ions from conventional IMAC medium. Ni Smart Beads 6FF is stable in all buffers commonly used in IMAC.


Ni Smart Beads 6FF Gravity Column are packed with 1ml and 5ml of Ni Smart Beads 6FF. It is easy to operate.

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• Product Description

Item Description
MatrixHighly cross-linked 6% agarose
Loading capacity>10 mg 6XHis-tagged protein/mL matrix
Microsphere particle size45–165 μm
Maxi pressure0.3 MPa, 3 bar
Storage buffer1XPBS containing 20% ethanol
Storage temperature2-8℃


• Related Products

Product Cat.No.Size
Ni Smart Beads 6FF Gravity ColumnSA036GC011 ml
SA036GC055 ml


• Download Instructions

Ni Smart Beads 6FF Gravity Column 2024.09.pdf



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