Ni Smart Beads is a new immobilized metal ion affinity chromatography (IMAC) medium precharged with nickel ions. Ni Smart Beads is designed mainly for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants. The strong nickel ion binding also provides very high resistance to EDTA and reducing agents like DTT. Ni Smart Beads enable direct loading of large sample volumes without having to remove agents that cause stripping of nickel ions from conventional IMAC medium. Ni Smart Beads is stable in all buffers commonly used in IMAC.
Ni Smart Beads Gravity Column are packed with 1ml and 5ml of Ni Smart Beads . It is easy to operate.
• Product Description
| Item | Description |
| Matrix | 4% agarose |
| Loading capacity | >10 mg 6×His-tagged Protein/ml matrix |
| Microsphere particle size | 45-165 μm |
| Maxi pressure | 0.1 MPa, 1 bar |
| Storage buffer | 1× PBS containing 20% ethanol |
| Storage temperature | 2-8℃ |
• Related Products
| Product | Cat.No. | Size |
| Ni Smart Beads Gravity Column | SA035GC01 | 1 ml |
| SA035GC05 | 5 ml |
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