Con A Beads 4FF is an affinity chromatography medium that conjugates Con A with agarose to purify some glycoproteins. Con A is a plant haemagglutinin separated from giant bean(Jack bean, Canavalia ensiformis),which can binds ɑ - D - pyran mannose, ɑ - D - pyran on glucose and Molecular groups related to its space position of the combination.Con A binds to carbohydrate molecules mainly in the hydroxyl part of C-3, C-4 and C-6. Con A combines better with D-pyranoid mannose than with D-pyranosaccharide.Con A Beads 4FF is used to isolate and purify some glycoproteins, membrane proteins, sugar lipids, polysaccharides, membrane vesicles with mannitin or glucoside residues, lgM, hormone lipoproteins and so on. Substances such as trypsin inhibitors in human serum, alkaline phosphatase, calf spleen phosphodiesterase, various variants of alpha-fetoglobulin and certain hormones such as chorionic gonadotropin (HCG) and luteinizing hormone (LH) can be purified with it.
PreCap Con A is one of a range of prepacked, ready-to-use columns for affinity chromatography. It is packed with 1 ml and 5 ml of Con A Beads 4FF. Five different packing sizes are available. PreCap Con A has the standard interface and can be adapted to all kinds of chromatography system, such as ÄKTA. It is fast, simple and easy operation.
• Product Description
| Item | Description | |
| Size | 1 ml | 5 ml |
| Column size | 0.7×2.5 cm | 1.6×2.5 cm |
| Matrix | Highly cross-linked 4% agarose | |
| Ligand | Con A | |
| Binding capacity | >20mg thyroglobulin /ml medium | |
| Particle size | 45-165 μm | |
| Maxi pressure | 0.3 MPa,3 bar | |
| pH stability | 4-9 | |
| Srorage buffer | 0.1 M acetate,1 M NaCl,1 mMCaCl2,1 mM MnCl2,1 mM MgCl2, 20% ethanol, pH 6.0 | |
| Storage temperature | 2 – 8℃ | |
• Related Products
| Product | Cat.No. | Size |
| Con A Beads 4FF | SA028005 | 5 ml |
| SA028025 | 25 ml | |
| SA028100 | 100 ml | |
| SA028500 | 500 ml | |
| SA02801L | 1 L | |
| SA02810L | 10 L | |
| PreCap Con A | SA027C11 | 1X1 ml |
| SA028C15 | 1×5 ml | |
| SA028C51 | 5×1 ml | |
| SA028C55 | 5X5 ml | |
| SA028CS | 3X1 ml+1X5 ml |
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