Smardex G-25 is a gel filtration medium prepared by cross-linked dextran. Size exclusion chromatography (SEC), also called Gel filtration (GF), separates molecules on the basis of differences in size as they pass through a SEC medium packed in a column. Media for SEC contains spherical particles with pores of different sizes where molecules smaller than the pores are retarded relative to molecules larger than the pores. Samples are eluted by a single buffer without gradients. Buffer conditions can be varied to suit the sample type or the requirements for the next purification, analysis, or storage step.
Smartdex G-25 is well established for desalting, removing contaminants and buffer exchange in industrial applications.
• Product Description
| Item | Smartdex G-25 Coarse | Smartdex G-25 Medium | Smartdex G-25 Fine |
| Average particle size,wet D50(μm) | 200-250 | 120-140 | 70-90 |
| Fractionation range[Mr] Dextran | 1×102-5×103 | 1×102-5×103 | 1×102-5×103 |
| Fractionation range[Mr] Globular Proteins | 1×103-5×103 | 1×103-5×103 | 1×103-5×103 |
| pH stability, operational | 2-13 | 2-13 | 2-13 |
| Swelling factor | 4-6 ml/g dry beads | 4-6 ml/g dry beads | 4-6 ml/g dry beads |
| Maxi flow velocity | - | 150 cm/h | 60 cm/h |
Storage: Dry Smartdex G-25 should be stored at 4℃ to 30℃.
Packed columns and used gel should be stored in 0.02% NaN3 at 4℃.
• Related Products
| Product | Cat.No. | Size |
| Smartdex G-25 Coarse | SEC001S2 | 25 g |
| SEC0011 | 100 g | |
| SEC0015 | 500 g | |
| SEC0016 | 1 Kg | |
| SEC0017 | 10 Kg | |
| Smartdex G-25 Medium | SEC003S2 | 25 g |
| SEC0031 | 100 g | |
| SEC0035 | 500 g | |
| SEC0036 | 1 Kg | |
| SEC0037 | 10 Kg | |
| Smartdex G-25 Fine | SEC005S2 | 25 g |
| SEC0051 | 100 g | |
| SEC0055 | 500 g | |
| SEC0056 | 1 Kg | |
| SEC0057 | 10 Kg |
• Download Instructions
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