Smart-Lifesciences rProtein A Beads 4FF is an affinity chromatography medium designed for the purification of monoclonal antibody, polyclonal antibody and Fc tag fusion protein.The recombinant protein A is coupled to highly cross-linked 4% sepharose gel. This coupling method can improve the binding capacity for immunoglobulin. The static binding capacity of rProtein A Beads 4FF is greater than 40 mg human IgG/ml settled beads. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc. The characteristics of rProtein A Beads 4FF are summarized in Table .
Protein A ,a bacterial cell wall protein isolated from Staphylococcus aureus, combines mammalian IgGs mainly through Fc regions. Recombinant protein A contains five high affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding.
rProtein A Beads 4FF Gravity Column are packed with 1ml and 5ml of rProtein A Beads 4FF . It is easy to operate.
• Product Description
| Item | Description |
| Matrix Spherical | Highly cross-linked 4% agarose |
| Ligand | Recombinant Protein A |
| Static binding capacity | >40 mg Human lgG/ml medium |
| Particle size | 45-165 μm |
| Maxi Pressure | 0.3 MPa, 3 bar |
| PH stability | 3-10 |
| Storage solution | 1X PBS containing 20% ethanol |
| Storage temperature | 2 – 8℃ |
• Related Products
| Product | Cat.No. | Size |
| rProtein A Beads 4FF Gravity Column | SA015GC01 | 1 ml |
| SA015GC05 | 5 ml |
• Download Instructions
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