siRNA
03/06/2024Currently, nucleic acid drugs are mainly purified using reversed-phase chromatography or ion-exchange chromatography.
Based on the principle of the difference between stationary and mobile phases,HPLC is an efficient purification method.Depending on the characteristics of the oligonucleotide, a suitable column can be selected, such as RP-HPLC or IE-HPLC. For RP-HPLC, the hydrophobic surface in the solid phase can well bind the oligonucleotides containing the 5'-DMT protecting group, which can achieve a good separation effect.
On the other hand, anion-exchange chromatography can be combined with negatively charged oligonucleotides. By adjusting the elution intensity, impurities can be removed,effectively.
The process steps are as follows: solid phase synthesis → HIC → detrityl methylation → IEX → ultrafiltration → drug substance.
